Magnum primers are designed to make more gas than standard primers. This is because, for best burning, powders need a minimum starting pressure, and when a case gets larger than normal (magnum) it takes more gas to reach that pressure. Hence, magnum primers.
In addition to the pressure difference, in 1989 CCI changed the primer mix formulation to better ignite the 1960's era spherical powder formulations, whose heavy outside deterrent coatings can cause them to need not only higher start pressure to ignite consistently, but a hotter spark, so metal particles were added to make more thrown sparks. Alan Jones once worked for CCI and reported that in
this article. So they may be used where their added start pressure is not required, but the sparks are.
Reloadron,
Call CCI back and ask if the amount and type of priming mix in the 550 and 400 are the same? One fellow reported talking to them and having them say that it was. That doesn't mean the primer cups are the same thickness, though. I would expect the rifle types to be thicker as rifle firing pins generally strike harder than a lot of revolvers do and typical pressures are higher. But if that reported information is correct, then a handgun that hit hard enough to produce reliable ignition with the 400's could use them in place of 550's. Assuming the cups are different, I would hesitate to try the reverse out of concern for getting a pierced primer.
One of the things Alan Jones reported was that primer design changes more frequent than people realize, so it is possible for something to be true at one time, then untrue a couple of years later. One has to keep updating one's information, alas.