This is NOT recommended unless you have the special tooling.
To disassemble, you should use a special wrench to unscrew the ejector.
This allows the cylinder to be removed from the crane assembly.
To remove the ejector rod, a special tubular spanner is used to unscrew a bushing from the rear of the crane.
Looking at the rear end of the crane assembly, there is a bushing inside the rear end of the crane.
You need the tubular spanner that slips inside the crane and around the ejector rod.
This tool is available from Brownell's:
http://www.brownells.com/aspx/NS/store/ProductDetail.aspx?p=712&title=COLT+CRANE+BUSHING+TOOL&s=2894
NOTE: This tool MAY not fit the larger New Service assembly.
It's possible to make a wrench from HARDENED tubular steel.
WARNING: Cylinder disassembly and reassembly is VERY easy to have problems with.
You should have used a special ejector wrench to unscrew the ejector.
Without the wrench be VERY VERY careful when replacing the ejector.
These are extremely easy to cross thread, and if you do, the assembly is ruined.
Since parts are hard to find now, and there are few pistolsmiths around who still work on the big Colt's, a damaged cylinder assembly is going to be extremely expensive to repair, and it isn't going to be easy to find a repairman.
No BS, on the old Colt's, cylinder disassembly is one of those ways to ruin a gun very quickly.