Powder will not burn differently in different barrel lenghts. It may not have a complete burn in a shorter barrel, but the burn rate is not affected.
A bullet is generally stabilized by the time it has passed thru the first inch of rifling. The added barrel length with the extra rifling will not increase any stabilization. Once a bullet has stabilized it remains that way except when it crosses the speed of sound or when it's velocity drops below a certain point (provided of course it doesn't hit anything). A longer heavier bullet will hold it's stabilization longer as it will shed it's velocity slower than a shorter lighter bullet, but the amount of rifling has nothing to do with this. The amount of rifling per inch will affect the stabilization of a bullet depending upon it's weight. A heavy for caliber bullet stabilizes in a faster twist.
Velocity increase with barrel lengths depends on complete burn of the powder and the burn rate of the powder. A faster burning powder's velocity usually will not equal a slower burning powder in a longer barrel and the opposite in a shorter barrel. Once the fast burning powder has completely burned well inside the barrel no more appreciable pressure is generated.
POI will be affected by the longer heavier barrel as well with bullet weight and bullet velocity as all will be a factor in the recoil and when the bullet leaves the barrel, but that has no effect on the grouping of several shots.
Try slower burning powders and heavy bullets (which has a longer bearing surface). That will help with long range shooting. If your shooting is going to be at the 25 yard mark then a faster burning powder may help some.
Without knowing the loads you tried then it's hard for someone to recommend adjustments. Also every firearm is an individual. What may work in one does not mean it will work in the next.
Handloaded accuracy will depend on what powder is used (even different lots can make a difference) and how much. What weight bullet is used, it's bearing surface, it's nose configuration, the amount of crimp, and it's seating depth. The brand of case (due to volume differences) and a consistant trim length is required. Even brands and type of primer will affect accuracy. Most important is consistancy from load to load to allow the POI to be repeatable. All of this have to be experimented with to find what your particular barrel prefers.
Then the next biggest factor comes from the shooter and the shooting technique. Are you shooting offhand or benching your revolver? If benching then what is your benching technique? Are you familar with the revolver and the cartridge? Is your eyesight and shooting technique up to the task? By not knowing you or how you shoot these are also questions to consider. I'm just trying to point out that you are also part of this equation.